CV

Education

2011 - 2016
Ph.D. in Structural Biology, Weizmann Institute of Science.

2008 - 2011
M.Sc. in Structural Biology, Weizmann Institute of Science.

2005 - 2008
B.Sc. in Chemistry, Bar-Ilan University, graduated magna cum laude.

Research experience

Postdoc, Stanford University
Laboratory of Joseph Puglisi and Elisabetta Viani Puglisi.
Research focus: Architecture of HIV viral RNA.

Ph.D., Weizmann Institute of Science
Adviser: Ada Yonath.
Thesis: The Origin of the Ribosome and its Paralyzation by Antibiotics.

M.Sc., Weizmann Institute of Science
Adviser: Ada Yonath.
Thesis: Towards the Determination of the Structure of Mycobacterium smegmatis Ribosome.

Rotations at Weizmann Institute of Science
1) Ribosome structure and function, laboratory of Ada Yonath.
2) Organized lipid domains, laboratory of Lia Addadi.
3) Nano and bio composite materials, laboratory of Daniel Wagner.

Undergraduate research at Bar Ilan University
Conducting polymers, laboratory of Joseph Frey.

Publications

Advances in understanding the initiation of HIV-1 reverse transcription
Krupkin M^*, Jackson LN^*, Ha B^*, Puglisi EV.
Current Opinion in Structural Biology, Volume 65, December 2020, Pages 175-183.
link.

Structural insights of lincosamides targeting the ribosome of Staphylococcus aureus
Matzov D^*, Eyal Z^*, Benhamou RI, Shalev-Benami M, Halfon Y, Krupkin M, Zimmerman E, Rozenberg H, Bashan A, Fridman M, Yonath A.
Nucleic Acids Res. 2017 Sep 29;45(17):10284-10292.
link.

The ribosomal protein uL22 modulates the shape of the protein exit tunnel
Wekselman I, Zimmerman E, Davidovich C, Belousoff M, Matzov D, Krupkin M, Rozenberg H, Bashan A, Friedlander G, Kjeldgaard J, Ingmer H, Lindahl L, Zengel JM, Yonath A.
Structure. 2017 Aug 1;25(8):1233-1241.e3. Epub 2017 Jul 6.
link.

Avilamycin and evernimicin induce structural changes in rProteins uL16 and CTC that enhance the inhibition of A-site tRNA binding
Krupkin M^*, Wekselman I^*, Matzov D, Eyal Z, Diskin Posner Y, Rozenberg H, Zimmerman E, Bashan A, Yonath A.
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6796-E6805.
link.

A novel pleuromutilin antibacterial compound, its binding mode and selectivity mechanism
Eyal Z^*, Matzov D^*, Krupkin M, Paukner S, Riedl R, Rozenberg H, Zimmerman E, Bashan A, and Yonath A.
Sci Rep. 2016 Dec 13;6:39004.
link

Ribosomal antibiotics: Contemporary challenges
Auerbach-Nevo T, Baram D, Bashan A, Belousoff M, Breiner E, Davidovich C, Cimicata G, Eyal Z, Halfon Y, Krupkin M, … Yonath A.
Antibiotics (Basel). 2016 Jun 29;5(3). pii: E24.
link

Structural insights into species-specific features of the ribosome from the pathogen staphylococcus aureus
Eyal Z^*, Matzov D^*, Krupkin M, Wekselman I, Paukner S, Zimmerman E, Rozenberg H, Bashan A, Yonath A.
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):E5805-14.
link

A recombinant collagen–mRNA platform for controllable protein synthesis
Sun L, Xiong Y, Bashan A, Zimmerman E, Shulman Daube S, Peleg Y, Albeck S, Unger T, Yonath H, Krupkin M, Matzov D, Yonath A.
Chembiochem. 2015 Jul 6;16(10):1415-9.
link

Chapter 6: Glimpse into the origin of life: What was first, the genetic code or its products, the proteins?
Krupkin M, Bashan A, Yonath A.
“Why does Evolution Matter? The Importance of Understanding Evolution”, G. Trueba, ed. (Cambridge Scholars Publishing),(2014), p. 87-100.
link

Protoribosome by quantum kernel energy method
Huang L, Krupkin M, Bashan A, Yonath A, Massa L.
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14900-5.
link

A vestige of a prebiotic bonding machine is functioning within the contemporary ribosome
Krupkin M, Matzov D, Tang H, Metz M, Kalaora R, Belousoff MJ, Zimmerman E, Bashan A, Yonath A.
Philos Trans R Soc Lond B Biol Sci. 2011 Oct 27;366(1580):2972-8.
link.

The ribosome as drug target: lessons from 3D structures
Bashan A, Zimmerman E, Belousoff MJ, Rozenberg H, Davidovich C, Wekselman I, Shapira T, Krupkin M, Yonath A.
Isr Chem Soc. 2010, 25, 10-18.

The proto-ribosome : An ancient nano-machine for peptide bond formation
Davidovich C, Belousoff M, Wekselman I, Shapira T, Krupkin M, Zimmerman E, Bashan A, Yonath A.
Isr J Chem. 2010 Jun 18;50(1):29-35.

^* Equal contribution.

Conferences and Presentations

2019

Guest seminar: Layers of Protection: from molecular mechanisms to organism defense. School of medicine, Stanford.
Seminar: Avilamycin Induces Structural Changes In Ribosomal Proteins uL16 And CTC That Enhance The Inhibition Of A-Site tRNA Binding. Simons electron microscopy center, New York Structural Biology Center.
Guest seminar: Layers of Protection: from molecular mechanisms to organism defense. Biochemistry and Biophysics department, UCSF.
Guest seminar: Layers of Protection: from molecular mechanisms to organism defense. Chemistry department, University of Utah.

2016

Guest seminar: Origin of The Ribosome and its Paralyzation by Antibiotics. Biological engineering department, MIT.
Talk: The origin of the ribosome: A vestige of a prebiotic bonding machine is functioning within the contemporary ribosome. Israel Society for Astrobiology and the Origin of Life (ILASOL), 29th annual meeting, Ben-Gurion University, Israel.
Talk: Avilamycin induces structural changes in rProteins uL16 and CTC that enhance the inhibition of A-site tRNA binding. 8th Graduate Students Chemistry Symposium, Ben-Gurion University, Israel.
Talk: The structure of avilamycin bound to the large ribosomal subunit. Israel Crystallography Association Meeting ICA2016, Tel-Aviv University, Israel.
Poster: The structure of avilamycin bound to the large ribosomal subunit. Ribosome Structure and Function EMBO conference, Strasbourg, France.

2015

Oral: The origin of the ribosome: A vestige of a prebiotic bonding machine is functioning within the contemporary ribosome. Genetics, Genomics and Evolution conference, Tel-Aviv University, Israel. Best Talk Award

2014

Poster: The origin of the ribosome: A vestige of a prebiotic bonding machine is functioning within the contemporary ribosome. The Society for Molecular Biology and Evolution SMBE2014 meeting, San Juan, Puerto Rico.
Poster: The origin of the ribosome: A vestige of a prebiotic bonding machine is functioning within the contemporary ribosome.The RNA society 19th annual meeting, Quebec, Canada.
Poster: The origin of the ribosome: A vestige of a prebiotic bonding machine is functioning within the contemporary ribosome. Structure and dynamics of RNA interactions - 4th Annual Summer Symposium, Montreal, Canada.

2013

Talk: The Ribosome’s Origin – The Proto Ribosome. RNA Bioinformatics Structure Function and Regulation workshop, Technion, Israel.
Guest seminar: The Ribosome’s Origin – The Proto Ribosome. School of medicine, Stanford, USA.
Poster: The Ribosome’s Origin – The Proto Ribosome. Ribosomes, Napa, USA.

2012

Talk: “A vestige of a prebiotic bonding entity is functioning within the contemporary ribosome”. Israel Society for Astrobiology and the Origin of Life (ILASOL), 26th annual meeting, Weizmann Institute of Science, Israel.

2011

Poster: “Mycobacterium Smegmatis Ribosome as A Tool for A Structural Insight into Antibiotics Action on Pathogens”. ILANIT, Federation of the Israel Societies for Experimental Biology, Eilat, Israel.
Seminar: “Towards the Determination of the Structure of Mycobacterium smegmatis Ribosome”. Structural Biology Department, Weizmann Institute of Science, Israel.

2010

Talk: “Towards the Determination of the Structure of Mycobacterium smegmatis Ribosome”.BCA/CCP4 Summer School in Protein Crystallography, Oxford, UK

Awards and Fellowships

2016: Travel fellowship award, Cincinnati Children’s Hospital Medical Center, Ohio, USA.
2015: Best talk, Genetics, Genomics and Evolution conference, Tel Aviv University.
2013: Vallee travel fellowship award, “The 63rd Lindau Nobel Laureate Meeting”, Germany.
2012 - 2016: Adams Ph.D. fellowship of the Israel Academy of Sciences and Humanities.
2007: Schächter summer scholarship for research, Bar-Ilan University.
2006: Dean’s Honors list, Bar-Ilan University.

Other Activities

2020

S2C2 SLAC cryoEM Image Processing workshop, Stanford, USA.
Retroviruses 2020 Cold Spring Harbor Laboratory Meeting, NY.
“Conference on Retroviruses and Opportunistic Infection- CROI 2020“, Boston, USA.
Reviewer board for the international peer-reviewed journal Antibiotics.